Endocrine Abstracts

Epigenetic deregulation is emerging as a component of the parathyroid tumorigenesis. We identified a long non-coding RNA (LNCRNA) signature clearly distinguishing parathyroid adenomas (PAds) and carcinomas from normal glands. Among the deregulated LncRNAS, HAR1B was upregulated in PAds harboring the common chromosome 11q loss of heterozygosity (Chr11q-LOH; n=10) compared with PAds harboring normal chromosome 11 haplotype (Chr11q-WT; n=12; P&...

Parathyroid carcinomas (PCas) are rare endocrine malignant neoplasia characterized by an abnormal PTH secretion and, consequently, severe and uncontrolled hypercalcemia. Clinically, the preoperative diagnosis can be misleading, because PCas share some molecular and clinical similarities with benign lesions and are often indolent. The histological diagnosis of carcinoma is achieved based on the presence of mitotic nuclear figures, capsular invasion, parenchyma infiltration, vas...

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Long noncoding RNAs (LncRNAs) are transcripts of more than 200 nucleotides not translated into proteins. They carry out diverse functions, including transcriptional regulation in cis or trans, organization of nuclear domains, and regulation of proteins or RNA molecules. Aberrant expression of LncRNAs has been reported in human cancers; indeed, data in parathyroid tumors are lacking. Based on the profiling through lncProfilersTM qPCR Array with SYBR...

Aberrant epigenetic features occurring in parathyroid tumors involve DNA methylation, histone methylation, and non-coding RNAs. RASSF1A and APC were frequently downregulated in human cancers. Here, we investigated RASSF1A and APC methylation status in a series of parathyroid tumors from Italian patients with primary hyperparathyroidism (PAds, n=80), confirming RASSF1A and APC promoter methylation as a hallmark of sporadic parathyroi...

The Hippo pathway is involved in human tumorigenesis and regeneration. Here, we investigated the Hippo co-activator YAP1 and the kinase LATS1/2 in tumors of the parathyroid glands, which are almost invariably associated with primary hyperparathyroidism. Compared with normal parathyroid glands (n = 3), where YAP1 was detectable in the cytoplasm and in the nucleus, parathyroid adenomas (PAds; n = 10) had YAP1 accumulation mainly at the nuclear level, while in p...